Blood Genomic DNA Extraction Kit,IC-5335
(For Research Use Only)
Product Description
The Blood Genomic DNA Extraction Kit provides a rapid, efficient, and reliable method for isolating high-quality genomic DNA from whole blood, plasma, serum, or other blood-derived samples. The kit utilizes a silica-membrane-based spin column technology, ensuring high DNA yield and purity suitable for downstream applications such as PCR, sequencing, genotyping, and molecular diagnostics.
Product Specifications
- Sample Type: Whole blood, plasma, serum, buffy coat
- Starting Volume: 100ul – 1 mL of whole blood
- Average Yield: 4–20ug DNA per 200ul of whole blood
- Elution Volume: 50–200ul (adjustable)
- Purity (A260/A280): 1.7–1.9
- Storage: Room temperature (15–25℃) for 12 months
Key Features
✔ High Efficiency: Optimized lysis buffer ensures complete cell lysis and protein removal.
✔ Fast Procedure: Complete extraction in 30–60 minutes (depending on sample number).
✔ High Purity: Removes PCR inhibitors, hemoglobin, and other contaminants.
✔ Scalable: Compatible with manual or automated systems.
✔ Stable Output: DNA is ready for long-term storage or immediate use.
Protocol Summary
1. Sample Lysis
Mix 100–500ul whole blood with 500ul Lysis Buffer and incubate at 60℃for 10 min.
Centrifuge briefly to pellet debris.
2. Protein Removal
Add 200ul Protein Precipitation Buffer, vortex, and centrifuge at 12,000 rpm for 5 min.
3. DNA Binding
Transfer supernatant to a spin column and centrifuge at 10,000 rpm for 1 min. Discard flow-through.
4. Washing
Add 500ul Wash Buffer and centrifuge. Repeat twice.
5. Elution
Add 50–200ul Elution Buffer (preheated to 70℃), incubate for 2 min, and centrifuge to collect DNA.
Precautions
1.Use gloves and protective equipment to avoid contamination.
2.Ensure complete lysis by thorough mixing.
3.Do not exceed recommended centrifugation speeds.
4.Store buffers at room temperature; precipitates may form but can be dissolved at 37℃.
5.For long-term storage, keep DNA at -20°C.
Ordering Information
|
Cat./REF. |
Size |
Price($） |
Price(�) |
Price(￥/CNY） |
Price(￥/JYP） |
|
IC-5335-050 |
50Tests |
$110.00 |
� 132.00 |
￥1,100.00 |
￥21,450.00 |
|
IC-5335-100 |
100Tests |
$185.00 |
� 222.00 |
￥1,850.00 |
￥36,075.00 |
|
IC-5335-200 |
200Tests |
$555.00 |
� 666.00 |
￥5,550.00 |
￥108,225.00 |
