Streptavidin Magnetic Beads (1um),IC-8112

Product Introduction  

The streptavidin-biotin (SA-Biotin) system exhibits extremely high binding affinity (\(K=10^{-15}\)) and is widely used in biomolecular separation and detection. These magnetic beads are covalently conjugated with streptavidin (SA) on superparamagnetic microspheres, enabling efficient binding to biotinylated antibodies, nucleic acids, proteins, and other molecules.  The beads feature uniform particle size (1 um), well-defined morphology, and rapid magnetic response, making them suitable for immunoprecipitation, nucleic acid purification, cell sorting, and high-throughput automated applications.  

Features  

High Binding Capacity:Biotinylated nucleic acid probe binding capacity: ¡Ý450 pmol/mg beads ,Biotinylated antibody/protein binding capacity: ¡Ý15 ug/mg beads  

Excellent Stability :Supplied in PBS buffer containing 0.1% BSA and 0.1% proclin-300, stable for 2 years at 4¡ãC.  

Fast Magnetic Response :Superparamagnetic microspheres enable rapid magnetic separation within 1 minute.  

Broad Applicability:Suitable for immunoassays, nucleic acid purification, DNA-protein interaction studies, cell sorting, and more.  

Usage Instructions  

1 Buffer Preparation  

1.1 Buffer I (For Nucleic Acid Binding)  

- 10 mM Tris-HCl (pH 7.5)  

- 1 mM EDTA  

- 1 M NaCl  

- 0.01%~0.1% Tween-20  

1.2 Buffer II (For Antibody/Protein Binding)  

- PBS (pH 7.4)  

- 0.05% Tween-20  

- Optional: 0.01%~0.1% BSA  

2 Binding Biotinylated Nucleic Acids  

2.1 Take 100 uL of beads, vortex for 20 s, and separate using a magnet for 1 min. Discard the supernatant.  

2.2 Wash twice with 1 mL Buffer I to remove storage solution.  

2.3 Add 500 uL of biotinylated nucleic acid diluted in Buffer I (final concentration 2 mg/mL) and rotate at room temperature for 30 min.  

2.4 Perform magnetic separation, collect the supernatant (optional for binding efficiency analysis), wash the beads 3 times, and resuspend in low-salt buffer for downstream use.  

3 Binding Biotinylated Antibodies/Proteins  

3.1 Take 100 uL of beads, perform magnetic separation, and discard the supernatant. Wash twice with Buffer II.  

3.2 Add 1 mL of biotinylated antibody/protein diluted in Buffer II (final concentration 1 mg/mL) and rotate at room temperature for 60 min.  

3.3 Perform magnetic separation, discard the supernatant, wash 3 times, and resuspend in Buffer II for further use.  

4 Magnetic Particle Chemiluminescence Immunoassay (96-Well Plate Protocol)  

4.1 Add 50 uL of beads (0.2-0.8 mg/mL) per well, perform magnetic separation, and discard the supernatant.  

4.2 Add 100 uL of biotinylated capture antibody and incubate at 37¡ãC for 15 min. Wash 3 times.  

4.3 Add 50 uL of sample and incubate at 37¡ãC for 15 min. Wash 3 times.  

4.4 Add 100 uL of enzyme-labeled antibody and incubate at 37¡ãC for 15 min. Wash 3 times.  

4.5 Add 150 uL of substrate solution, incubate in the dark for 5 min, and measure chemiluminescence.  

5 Biotin-Bead Dissociation (Optional)  

5.1 Method 1: Boil in 0.1% SDS for 5 min.  

5.2 Method 2: Incubate in 95% formamide (with 10 mM EDTA, pH 8.2) at 65¡ãC for 5 min or 90¡ãC for 2 min.  

Precautions  

1.Storage & Handling  

- Store at 4¡ãC. Avoid freezing or repeated freeze-thaw cycles.  

- Vortex thoroughly before use to ensure uniform suspension.  

2.Optimization Recommendations  

- Recommended biotinylated molecule input: 1-2¡Á the bead binding capacity for saturation.  

- Binding efficiency may vary with molecular size; optimize conditions accordingly.  

3.Minimizing Bead Loss  

- Magnetic separation time should be ¡Ý1 min for complete capture.  

- Use low-retention pipette tips to minimize bead loss.  

4. Safety Notes  

- For research use only. Not for clinical, diagnostic, or therapeutic applications.  

- Wear lab coats and gloves during handling.

Order Information

Note: This manual provides general guidelines. Adjust experimental conditions as needed.