DAPI Staining Solution (1 mg/mL),IC-20017

Description  

DAPI (4¡ä,6-Diamidino-2-Phenylindole) is a blue-fluorescent DNA stain widely used in fluorescence microscopy and flow cytometry for nuclear staining. This solution is provided at a concentration of 1 mg/mL in ultrapure water, ensuring consistent performance and ease of use in various applications, including fixed and permeabilized cell preparations.  

Key Features  

- High Affinity for DNA: Binds strongly to AT-rich regions in double-stranded DNA.  

- Bright Blue Fluorescence: Exhibits maximum excitation:358 nm,emission:461 nm.  

- Cell Membrane Impermeant: Suitable for staining fixed cells or permeabilized live cells.  

- Ready-to-Use Solution: Pre-diluted to 1 mg/mL for convenience.  

- Compatibility: Can be used alongside other fluorescent probes (e.g., FITC, TRITC) for multiplex assays.  

Usage

Sample Preparation  

1. Fix and permeabilize cells (if required) using appropriate methods (e.g., methanol/acetone fixation or detergent permeabilization).  

2. Wash cells with PBS (pH 7.4) to remove residual fixative.  

Staining Procedure  

1. Dilute DAPI stock solution (1 mg/mL) to a working concentration of 0.1¨C1 µg/mL in PBS or mounting medium.  

2. Apply the diluted solution to samples and incubate for 5¨C15 minutes at room temperature (protected from light).  

3. Rinse gently with PBS to remove excess dye.  

4. Mount samples with an antifade mounting medium (if required) and visualize using a fluorescence microscope with a DAPI filter set.  

Recommended Dilution  

- For most applications: 1:1000 to 1:2000 dilution (final concentration 0.5¨C1 µg/mL).  

- Adjust based on cell type and experimental conditions.  

Precautions  

1. Toxicity: DAPI is a known mutagen. Handle with gloves, protective clothing, and eye protection.  

2. Light Sensitivity: Protect from light during storage and use to prevent photobleaching.  

3. Disposal: Dispose of waste according to local regulations for hazardous chemicals.  

4. Storage: Store at 2¨C8¡ãC in the original container. Do not freeze. Stable for 12 months when stored properly.  

5. Compatibility: Avoid contact with strong oxidizers or acids.   

Product Statement  

This product is intended for research use only. For the storage of valuable samples, please conduct small-scale trials prior to large-scale experiments. After-sales service is limited to the product itself and does not entail any otherconsequential responsibility. Please be advised.  

This product is for research purposes only and is not intended for clinical diagnosis or treatment.  

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