Recombinant Human Ubiquitin(UBB)£¬REH-P0CG47P

Description

Ubiquitin is a multifunctional protein that can form covalently linked isopeptide bonds in various states, including mono- and polyubiquitin chains. Ubiquitin Protein, Human (His) is a recombinant human-derived ubiquitin protein, expressed by E. coli, with an N-terminal His tag.

Background

Ubiquitin exerts its effects by forming covalent isopeptide bonds with other proteins, appearing as monoubiquitin or polyubiquitin chains linked through specific Lys residues (e.g., K48, K63). The type of linkage dictates the substrate''''s fate: K48-linked chains typically target proteins for proteasomal degradation, while K63-linked chains are involved in signaling and endocytic trafficking. Free, unanchored polyubiquitin chains are also involved in activating protein kinases and other signaling events. The multifaceted role of ubiquitin underscores its importance in maintaining cellular homeostasis and regulating diverse biological pathways.

Product Information

- Species: Human

- Expression System: E. coli

- Tag: Tag-Free

- Accession Number: Met153-Gly288 (Accession # P0CG47)

- Gene ID: 7316

- Predicted Molecular Weight: 10.5 kDa

- Purity: ¡Ý 90%, as determined by reducing SDS-PAGE.

- Endotoxin Level: < 1.0 EU/¦Ìg, determined by the LAL method.

- Formulation:Supplied as a sterile solution in 20 mM Tris-HCl, pH 7.0, 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, with 10-25% glycerol.Note: For SPR assays, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Amino Acid Sequence

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRG

Verified Applications: Western Blot (WB), Enzyme Assay.

Storage and Stability

- Storage Conditions:

    - Stored at -80¡ãC for up to 1 year from the date of receipt.

    - It is stable at -20¡ãC for 3 months after opening.

    - It is recommended to freeze aliquots at -80¡ãC for extended storage. Avoid repeated freeze-thaw cycles.

- Shipping: Enzymes are highly recommended to be shipped at frozen temperature with dry ice.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Statement

For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Order Information