Streptavidin Magnetic Beads (2.8 µm), IC-8118
Product Introduction
This product consists of superparamagnetic microspheres (2.8 µm diameter) with streptavidin (SA) covalently conjugated to the solid-phase surface through robust protein coupling technology. Streptavidin, a tetrameric protein derived from Streptomyces avidinii, exhibits exceptionally high affinity for biotin (Kd ≈ 10⁻¹⁵ M), enabling efficient and specific capture of biotinylated antibodies, nucleic acids, proteins, and other ligand molecules. The magnetic beads feature uniform particle size, regular spherical morphology, and superparamagnetic properties, facilitating rapid magnetic separation and efficient target molecule capture. The strong streptavidin-biotin interaction ensures stable binding under a wide range of experimental conditions including varying pH, temperature, salt concentrations, and denaturing agents. This product is compatible with automated liquid handling systems for high-throughput applications and is suitable for immunodetection, nucleic acid isolation, protein-DNA interaction studies, and cell sorting.
Product Features
1. Excellent Biotin Binding Capacity: High-density streptavidin coating provides superior biotinylated molecule binding capacity with minimal non-specific binding.
2. Outstanding Stability: Streptavidin covalently coupled to the bead surface ensures minimal ligand leaching and consistent performance across diverse buffer conditions.
3. Rapid and Sensitive Magnetic Response: Superparamagnetic 2.8 µm microspheres enable fast magnetic separation with complete resuspension upon removal of the magnetic field.
4. Uniform Particle Size: Monodisperse beads with regular morphology ensure reproducible binding kinetics and consistent performance across applications.
5. Broad Experimental Compatibility: Suitable for a wide range of buffers, pH conditions, temperatures, and denaturing agents without loss of binding capacity.
Applications
1. Immunodetection and Protein Isolation: Specific capture of biotinylated antibodies or antigens for use as solid-phase carriers in immunoassays, ELISA, and immunoprecipitation.
2. Nucleic Acid Isolation and Probe Preparation: Specific binding of biotinylated nucleic acid probes for DNA and RNA hybridization experiments, sequencing library preparation, and nucleic acid purification.
3. DNA-Protein Interaction Studies: Capture of biotinylated target DNA or RNA fragments for studying protein-nucleic acid interactions including transcription factor binding, chromatin immunoprecipitation, and pull-down assays.
4. Cell Sorting and Activation: Immobilization of antibodies for specific recognition, isolation, and activation of target cells.
Specifications
Size: Available in 1 mL and 5 mL
Bead Diameter: 2.8 µm
Bead Type: Superparamagnetic microspheres
Surface Coating: Streptavidin covalently conjugated
Storage Buffer: 1×PBS containing 0.1% (w/v) BSA, 0.1% (v/v) ProClin-300
Binding Capacity: Dependent on the size and nature of the biotinylated molecule; end users should determine the capacity for specific biotinylated ligands.
Storage and Stability
Storage Conditions: Store at 4°C. Do not freeze.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed.
Protocol (For Reference Only)
Important: Do not freeze the magnetic beads. Before removing beads from the storage bottle, vortex thoroughly to ensure uniform suspension. Use high-quality pipette tips and reaction tubes to minimize bead and solution loss. The biotinylated molecule amount should be 1-2 times the bead binding capacity to ensure saturation.
1. Buffer Preparation
The following buffers are commonly used; salt concentration and pH may be adjusted as needed:
1) Buffer I (for binding biotinylated nucleic acids): 10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 1 M NaCl, 0.01%-0.1% Tween-20.
2) Buffer II (for binding biotinylated antibodies/proteins): PBS, pH 7.4, containing 0.05% Tween-20. Add 0.01%-0.1% BSA if needed.
3) Chemiluminescence Washing Buffer: Prepare according to requirements and equilibrate to room temperature before use.
2. Binding Biotinylated Nucleic Acids
1) Vortex the bead bottle for 20 seconds to resuspend the beads. Transfer 100 µL of beads to a new centrifuge tube using a pipette. Place the tube in a magnetic separator and let stand for 1 minute for magnetic separation. Aspirate the supernatant and remove the tube from the separator. Note: Calculate the required bead volume based on the amount of biotinylated molecules and the bead binding capacity specified in the product documentation. It is recommended to add biotinylated molecules at 1-2 times the bead binding capacity to achieve saturation.
2) Add 1 mL of Buffer I to the tube, close the cap, and vortex thoroughly to resuspend the beads. Perform magnetic separation and aspirate the supernatant. Note: If the bead volume used in step 1 exceeds 1 mL, add an equal volume of Buffer I.
3) Repeat step 2 once.
4) Add 500 µL of biotinylated nucleic acid diluted in Buffer I (to achieve a bead concentration of 2 mg/mL), vortex thoroughly to resuspend the beads. Place the tube on a rotator and incubate at room temperature for 30 minutes with continuous mixing.
5) Perform magnetic separation and transfer the supernatant to a new centrifuge tube for subsequent analysis.
6) Wash the beads three times following the procedure in step 2.
7) Resuspend the beads in an appropriate volume of low-salt buffer according to the requirements of the subsequent experiment. The biotinylated nucleic acid binding step is now complete, and the beads are ready for downstream applications.
8) The amount of nucleic acid bound to the beads can be calculated by measuring the nucleic acid concentration before and after the reaction: (Concentration before reaction - Concentration after reaction) × Reaction solution volume.
3. Binding Biotinylated Antibodies/Proteins
1) Vortex the bead bottle for 20 seconds to resuspend the beads. Transfer 100 µL of beads to a new centrifuge tube using a pipette. Place the tube in a magnetic separator and let stand for 1 minute for magnetic separation. Aspirate the supernatant and remove the tube from the separator. Note: Calculate the required bead volume based on the amount of biotinylated molecules and the bead binding capacity specified in the product documentation. It is recommended to add biotinylated molecules at 1-2 times the bead binding capacity to achieve saturation.
2) Add 1 mL of Buffer II to the tube, close the cap, and vortex thoroughly to resuspend the beads. Perform magnetic separation and aspirate the supernatant. Note: If the bead volume used in step 1 exceeds 1 mL, add an equal volume of Buffer II.
3) Repeat step 2 once, for a total of three washes.
4) Add 1 mL of biotinylated antibody/protein diluted in Buffer II (to achieve a bead concentration of 1 mg/mL), vortex thoroughly to resuspend the beads. Place the tube on a rotator and incubate at room temperature for 60 minutes with continuous mixing.
5) Perform magnetic separation and transfer the supernatant to a new centrifuge tube for subsequent analysis.
6) Wash the beads five times following the procedure in step 2.
7) Resuspend the beads in Buffer II or other buffer according to the requirements of the subsequent experiment. The biotinylated antibody/protein binding step is now complete, and the beads are ready for downstream applications.
4. Magnetic Microparticle Chemiluminescence Immunoassay
1) Adjust the bead concentration to an appropriate level (0.2-0.8 mg/mL recommended). Vortex for 20 seconds to resuspend the beads. Transfer 50 µL of beads to each well of a 96-well plate using a pipette. Perform magnetic separation, aspirate the supernatant, and remove the plate from the separator.
2) Add 100 µL of biotinylated capture antibody to each well, vortex thoroughly to resuspend the beads, and incubate at 37°C for 15 minutes. Perform magnetic separation, aspirate the supernatant, and remove the plate from the separator.
3) Add 200 µL of Washing Buffer to each well, vortex thoroughly to resuspend the beads, perform magnetic separation, and aspirate the supernatant. Repeat this step twice for a total of three washes.
4) Add 50 µL of analyte standard or test sample to each well, vortex thoroughly to resuspend the beads, and incubate at 37°C for 15 minutes. Perform magnetic separation, aspirate the supernatant, and remove the plate from the separator.
5) Add 200 µL of Washing Buffer to each well, vortex thoroughly to resuspend the beads, perform magnetic separation, and aspirate the supernatant. Repeat this step twice for a total of three washes.
6) Add 100 µL of enzyme-labeled detection antibody to each well, vortex thoroughly to resuspend the beads, and incubate at 37°C for 15 minutes. Perform magnetic separation, aspirate the supernatant, and remove the plate from the separator.
7) Add 200 µL of Washing Buffer to each well, vortex thoroughly to resuspend the beads, perform magnetic separation, and aspirate the supernatant. Repeat this step twice for a total of three washes.
8) Add 150 µL of substrate solution to each well, vortex thoroughly to resuspend the beads, and incubate for 5 minutes protected from light.
9) Read the 96-well plate using a chemiluminescence instrument and perform data processing.
5. Dissociation of Biotin and Streptavidin Magnetic Beads
If dissociation of biotinylated molecules from streptavidin magnetic beads is required, the following methods may be employed:
Method 1: 0.1% SDS, boil for 5 minutes.
Method 2: 10 mM EDTA, pH 8.2, containing 95% formamide, incubate at 65°C for 5 minutes or 90°C for 2 minutes. Dissociation efficiency is 95%.
Precautions
1. Avoid freezing the magnetic beads.
2. To minimize bead loss, each magnetic separation step should not be less than 1 minute.
3. Before removing beads from the storage bottle, vortex thoroughly to ensure uniform suspension. Avoid introducing air bubbles during handling.
4. Use high-quality pipette tips and reaction tubes to prevent loss due to bead and solution adhesion.
5. The size of the biotinylated molecule affects the bead binding capacity. End users should determine the binding capacity for specific biotinylated molecules experimentally.
6. Add biotinylated molecules at 1-2 times the bead binding capacity to ensure bead saturation.
7. This product is intended for scientific research by professionals only. Not for use in clinical diagnosis or treatment, not for use in food or drugs, and not to be stored in ordinary residences.
8. For your safety and health, wear laboratory protective clothing and disposable gloves when handling this product.
FAQ (Simplified)
Q1: What is the binding capacity of these magnetic beads?
A1: The binding capacity depends on the size and nature of the biotinylated molecule. End users should determine the capacity for specific biotinylated ligands experimentally. The amount of biotinylated molecule added should be 1-2 times the bead binding capacity to achieve saturation.
Q2: Can these beads be used with automated liquid handling systems?
A2: Yes. The uniform 2.8 µm particle size and superparamagnetic properties make these beads compatible with automated high-throughput platforms for immunodiagnostic and screening applications.
Q3: How should I store the magnetic beads?
A3: Store at 4°C. Do not freeze, as freezing may damage the bead structure and streptavidin coating. The product is stable for 24 months under recommended storage conditions.
Q4: How can I dissociate biotinylated molecules from the beads?
A4: Two methods are recommended: (1) 0.1% SDS, boiling for 5 minutes; or (2) 10 mM EDTA, pH 8.2, containing 95% formamide, incubate at 65°C for 5 minutes or 90°C for 2 minutes, with a dissociation efficiency of 95%.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of binding conditions is recommended for specific biotinylated molecules and experimental systems.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-8118
Product Name: Streptavidin Magnetic Beads (2.8 µm)
Size: 1 mL / 5 mL
Price:
1 mL: CNY ¥1800.00 / USD $180.00 / EUR �216.00 / JPY ¥32400.00
5 mL: CNY ¥5500.00 / USD $550.00 / EUR �660.00 / JPY ¥99000.00
2023 Version
