SDS-PAGE Protein Loading Buffer (5X), IC-7992
Product Introduction
This product is a 5X concentrated SDS-PAGE protein loading buffer formulated without the strong odor typically associated with traditional loading buffers containing ¦Â-mercaptoethanol. Protein loading buffer is an essential reagent for the preparation of protein samples prior to SDS-polyacrylamide gel electrophoresis, serving to denature proteins, impart negative charge, and provide a visible dye front for monitoring electrophoretic migration. This odorless formulation contains an alternative reducing agent that effectively reduces disulfide bonds while eliminating the unpleasant and potentially hazardous odor of thiol-based reducing agents. The buffer contains SDS for protein denaturation and charge normalization, glycerol for sample density to facilitate loading into wells, and a tracking dye to monitor migration progress. This product is suitable for use with standard Tris-Glycine SDS-PAGE gels and is compatible with subsequent Coomassie staining, Western blotting, and other downstream detection methods.
Product Features
1. Odorless Formulation: Contains an alternative reducing agent that eliminates the strong odor of traditional thiol-based reducing agents.
2. Ready-to-Use 5X Concentrate: Dilute with protein sample to a final 1X concentration for immediate use.
3. Effective Protein Denaturation: Complete denaturation and reduction of protein samples for accurate molecular weight separation.
4. Convenient Packaging: Available in multiple sizes with single-use aliquots for laboratory convenience.
5. Broad Compatibility: Compatible with standard SDS-PAGE gels and downstream staining or blotting procedures.
Specifications
Size: 5 mL (5 ¡Á 1 mL), 15 mL (1 ¡Á 15 mL)
Concentration: 5X
Form: Liquid
Storage and Stability
Storage Conditions: Store at -20¡æ for long-term storage. Working aliquots may be stored at 4¡æ for up to 3 months. Protect from light.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed.
Protocol (For Reference Only)
Important: Mix the loading buffer thoroughly before use to ensure uniform composition. Centrifuge briefly after thawing to collect contents at the bottom of the tube.
1. Sample Preparation: Mix protein sample with 5X SDS-PAGE Protein Loading Buffer at a ratio of 4:1 (sample:buffer) to achieve a final 1X concentration. For example, add 5 µL of 5X loading buffer to 20 µL of protein sample.
2. Denaturation: Heat the mixture at 95-100¡æ for 5-10 minutes to denature proteins.
3. Cooling and Loading: Cool the samples to room temperature, centrifuge briefly to collect condensation, and load directly onto SDS-PAGE gel.
4. Electrophoresis: Run the gel according to standard SDS-PAGE protocols until the dye front reaches the desired position.
Precautions
1. Vortex or shake the buffer before use, as components may precipitate during storage.
2. Heating time may be adjusted based on protein characteristics; membrane proteins or proteins with extensive disulfide bonds may require longer heating.
3. Avoid repeated freeze-thaw cycles for bulk packaging; aliquot if needed.
4. For certain membrane proteins or heat-sensitive proteins, incubation at 37¡ãC for 30 minutes is recommended. Published literature or technical documentation may also be consulted for specific protein sample preparation guidelines.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What makes this loading buffer odorless?
A1: This formulation contains an alternative reducing agent that replaces ¦Â-mercaptoethanol or DTT, eliminating the strong thiol odor while maintaining effective disulfide bond reduction.
Q2: Is this buffer compatible with Western blotting?
A2: Yes. The buffer is fully compatible with standard Western blotting procedures, including transfer to PVDF or nitrocellulose membranes and immunodetection.
Q3: How should I store this product?
A3: Store at -20¡ãC for long-term storage. After thawing, aliquots may be stored at 4¡ãC for up to 3 months. Protect from light.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of heating time and conditions may be required for specific protein samples.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-7992
Product Name: SDS-PAGE Protein Loading Buffer (5X), Odorless
Size: 5 mL (5 ¡Á 1 mL) / 15 mL (1 ¡Á 15 mL)
Suggested Retail Price:
5 mL (5 ¡Á 1 mL): CNY ¥230.00 / USD $23.00 / EUR €27.60 / JPY ¥4140.00
15 mL (1 ¡Á 15 mL): CNY ¥400.00 / USD $40.00 / EUR €48.00 / JPY ¥7200.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
