HRP-Beta Actin Antibody, PA-0060
Product Introduction
This product is a mouse monoclonal antibody directly conjugated to horseradish peroxidase (HRP) that recognizes endogenous levels of beta-actin protein. Beta-actin is a 42 kDa ubiquitously expressed cytoplasmic actin isoform that serves as a major component of the cytoskeleton and is involved in cell motility, structure, and integrity. As one of the most commonly used housekeeping proteins, beta-actin is widely employed as a loading control for Western blot normalization across diverse cell and tissue types. The HRP direct conjugation eliminates the need for a secondary antibody incubation step, streamlining the Western blot workflow and reducing non-specific background. This antibody has been validated for use in Western blot applications with reactivity against human, mouse, rat, monkey, chicken, pig, rabbit, and zebrafish samples.
Product Features
1. HRP Direct Conjugation: Eliminates the need for secondary antibody incubation, reducing protocol time and non-specific binding.
2. High Sensitivity: Recommended dilution range of 1:50,000 to 1:200,000 enables detection with minimal antibody consumption.
3. Broad Species Reactivity: Validated for human (H), mouse (M), rat (R), monkey (Mk), chicken (C), pig (P), rabbit (Rb), and zebrafish (Z).
4. Monoclonal Specificity: Affinity-purified monoclonal antibody provides consistent lot-to-lot performance with specific recognition of beta-actin.
Specifications
Size: Available in 50 µL and 100 µL
Host Species: Mouse
Clonality: Monoclonal
Conjugate: Horseradish Peroxidase (HRP)
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Beta-actin. The exact sequence is proprietary.
Purification: Affinity chromatography
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Specificity: Recognizes endogenous levels of Beta-actin protein.
Recommended Dilution: WB (1:50,000 - 1:200,000)
Gene Symbol: ACTB
Alternative Names: Actin cytoplasmic 1; Beta-actin
Entrez Gene (Human): 60 Entrez Gene (Mouse): 11461 Entrez Gene (Rat): 81822
SwissProt (Human): P60709 SwissProt (Mouse): P60710 SwissProt (Rat): P60711
Reactivity: Human (H), Mouse (M), Rat (R), Monkey (Mk), Chicken (C), Pig (P), Rabbit (Rb), Zebrafish (Z)
Applications: WB
Storage and Stability
Storage Conditions: Shipped at 4¡æ. Upon delivery, aliquot and store at -20¡æ for one year. Avoid freeze/thaw cycles.
Shelf Life: The product is stable for 12 months from the date of delivery when stored as directed.
Protocol (For Reference Only)
Important: Protect HRP-conjugated antibody from prolonged light exposure. Centrifuge the vial briefly before opening. The optimal working dilution should be determined empirically for each application.
1. Sample Preparation and Electrophoresis: Prepare protein samples and perform SDS-PAGE according to standard protocols. Transfer proteins to PVDF or nitrocellulose membrane.
2. Membrane Blocking: Block the membrane with 5% non-fat dry milk or BSA in TBST for 1 hour at room temperature.
3. Antibody Incubation: Dilute the Anti-Beta-actin Antibody-HRP Labeled at 1:50,000 to 1:200,000 in blocking buffer. Incubate the membrane with the diluted antibody for 1 hour at room temperature or overnight at 4¡æ with gentle agitation.
4. Washing: Wash the membrane 3-5 times with TBST for 5-10 minutes each.
5. Detection: Apply ECL or other HRP chemiluminescent substrate and image using a chemiluminescence imaging system. Beta-actin is detected as a band at approximately 42 kDa.
Precautions
1. Protect from prolonged light exposure to prevent HRP inactivation.
2. Avoid freeze/thaw cycles; aliquot upon delivery and store at -20¡æ.
3. The recommended dilution range is a guideline; optimal dilution must be determined empirically.
4. Sodium azide is included as a preservative; handle with appropriate precautions.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What are the advantages of an HRP-conjugated primary antibody?
A1: Direct HRP conjugation eliminates the need for a secondary antibody incubation step, reducing protocol time, potential cross-reactivity, and non-specific background.
Q2: What dilution should I use for Western blot?
A2: The recommended dilution range is 1:50,000 to 1:200,000. For initial experiments, a dilution of 1:100,000 is recommended. Optimize based on your specific sample and detection system.
Q3: What is the expected molecular weight of beta-actin?
A3: Beta-actin is detected as a band at approximately 42 kDa on SDS-PAGE.
Q4: Can this antibody be used for IHC or IF?
A4: This antibody has been validated for Western blot applications. For IHC or IF applications, validation in the specific experimental system is recommended.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of antibody dilution and experimental conditions is recommended.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: PA-0060
Product Name: Anti-Beta-actin Antibody-HRP Labeled
Size: 50 µL / 100 µL
Suggested Retail Price:
50 µL: CNY ¥1800.00 / USD $180.00 / EUR €216.00 / JPY ¥32400.00
100 µL: CNY ¥3050.00 / USD $305.00 / EUR €366.00 / JPY ¥54900.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
