Protein Ladder 10-180 kDa, Prestained Color, IC-8018
Product Introduction
This product is a prestained color protein ladder consisting of ten recombinant proteins with molecular weights ranging from 10 kDa to 180 kDa. The ladder is designed for use as a molecular weight reference standard in SDS-PAGE and Western blotting applications, allowing direct visualization of protein migration and transfer efficiency during electrophoresis and subsequent membrane transfer. The prestained bands provide immediate size estimation without the need for gel staining. The 10 kDa band is colored green (or light green) and the 70 kDa band is colored red, serving as easily identifiable reference markers for gel orientation and molecular weight estimation. This protein ladder is compatible with Tris-Glycine SDS-PAGE gel systems and is supplied in a ready-to-use loading buffer.
Product Features
1. Wide Molecular Weight Range: Covers 10 kDa to 180 kDa with ten distinct bands, suitable for size estimation of proteins across a broad range.
2. Two Color Reference Bands: Green (or light green) band at 10 kDa and red band at 70 kDa serve as distinctive orientation and reference markers.
3. Ready-to-Use Format: Supplied in loading buffer for direct loading onto SDS-PAGE gels without additional preparation.
4. Prestained for Visualization: Bands are visible during electrophoresis and on membranes after transfer, enabling real-time monitoring of protein migration and transfer efficiency.
5. Long-Term Storage Stability: Stable at -20¡ãC for 2 years.
Specifications
Size: Available in 250 µL and 10 ¡Á 250 µL
Band Sizes: 10, 16, 25, 32, 42, 55, 70, 95, 130, 180 kDa
Color Reference: 10 kDa band (green or light green), 70 kDa band (red)
Recommended Loading Volume: 5-10 µL per lane
Gel System: Tris-Glycine SDS-PAGE
Storage and Stability
Storage Conditions: Store at -20¡ãC for up to 2 years. Avoid repeated freeze-thaw cycles.
Shelf Life: The product is stable for 2 years from the date of manufacture when stored at -20¡ãC as directed.
Protocol (For Reference Only)
Important: Thaw the ladder at room temperature or 37¡ãC and vortex briefly before use. Do not heat the ladder before loading.
1. Thawing: Remove the ladder from -20¡ãC storage and allow to thaw at room temperature. Vortex briefly to ensure uniform mixing.
2. Loading: Load 5-10 µL of the prestained protein ladder directly onto the SDS-PAGE gel alongside protein samples. Do not heat the ladder before loading as this may cause protein aggregation.
3. Electrophoresis: Run the gel according to standard Tris-Glycine SDS-PAGE protocols. Monitor the migration of prestained bands during electrophoresis to estimate the separation of sample proteins.
4. Transfer: After electrophoresis, the prestained bands will transfer to PVDF or nitrocellulose membrane, allowing verification of transfer efficiency and molecular weight estimation on the blot.
Precautions
1. Store at -20¡ãC for long-term storage. Avoid repeated freeze-thaw cycles.
2. Do not heat the ladder before loading as this may cause protein aggregation and aberrant migration.
3. The recommended loading volume of 5-10 µL is a guideline; optimize based on gel thickness and detection sensitivity.
4. The apparent molecular weights of prestained proteins may differ slightly from unstained markers depending on the gel system used.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What are the reference band colors?
A1: The 10 kDa band is green (or light green) and the 70 kDa band is red, serving as orientation markers for gel loading and size estimation.
Q2: Should I heat the ladder before loading?
A2: No. Do not heat the protein ladder before loading as this may cause protein aggregation. Simply thaw at room temperature and vortex briefly before use.
Q3: What is the recommended loading volume?
A3: Load 5-10 µL per lane for standard mini-gel systems. Adjust volume based on gel thickness and required band intensity.
Q4: Is this ladder compatible with Western blotting?
A4: Yes. The prestained bands transfer to PVDF or nitrocellulose membranes, allowing molecular weight estimation directly on the blot.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of loading volume may be required for specific gel systems.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-8018
Product Name: Protein Ladder 10-180 kDa, Prestained Color
Size: 250 µL / 10 ¡Á 250 µL
Suggested Retail Price:
250 µL: CNY ¥350.00 / USD $35.00 / EUR €42.00 / JPY ¥6300.00
10 ¡Á 250 µL: CNY ¥3500.00 / USD $350.00 / EUR €420.00 / JPY ¥63000.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
