Premium Fetal Bovine Serum, IC-1902
Product Introduction
This product is a premium-grade fetal bovine serum (FBS) sourced from healthy bovine donors and processed under rigorous quality control standards. Fetal bovine serum is the most widely used serum supplement for cell culture, providing essential growth factors, hormones, attachment factors, transport proteins, and nutrients required for cell proliferation and maintenance. This premium-grade FBS is recommended for the culture of selected primary cells and the majority of tumor cell lines in laboratory research settings. Each batch undergoes comprehensive testing to ensure consistent cell growth performance with minimal lot-to-lot variability. The serum is sterile-filtered through multiple 0.1 µm membranes and tested for endotoxin levels, hemoglobin content, and the absence of bacteria, fungi, mycoplasma, and viruses.
Product Features
1. Premium Grade: Suitable for selected primary cells and most tumor cell lines in research applications.
2. Rigorous Quality Control: Each lot is tested for sterility, endotoxin, hemoglobin, and cell growth promotion.
3. Triple 0.1 µm Filtration: Sterile-filtered to ensure the absence of microbial contamination.
4. Comprehensive Testing: Tested negative for bacteria, fungi, mycoplasma, and viruses.
5. Consistent Performance: Lot-to-lot consistency verified by cell growth assays.
Specifications
Size: 500 mL
Source: Bovine
Grade: Premium
Sterilization: Triple 0.1 µm filtered
Recommended Applications: Selected primary cells and most tumor cell lines for laboratory research use only.
Storage and Stability
Storage Conditions: Store at -20°C for long-term storage. Once thawed, store at 4°C and use within 4-6 weeks. Avoid repeated freeze-thaw cycles.
Shelf Life: The product is stable for 5 years from the date of manufacture when stored at -20°C as directed.
Protocol (For Reference Only)
Important: Thaw serum properly before use. Do not thaw at temperatures above 37°C.
1. Thawing: Remove serum from -20°C storage and place at 4°C overnight to thaw. Alternatively, thaw in a 37°C water bath with gentle agitation until completely thawed. Do not leave at 37°C for extended periods.
2. Heat Inactivation (Optional): If required for specific cell types, heat inactivate the serum at 56°C for 30 minutes with gentle mixing. Swirl the bottle every 10 minutes to ensure uniform heating. Allow to cool to room temperature before use.
3. Aliquoting: For convenience, aliquot the thawed serum into single-use volumes and store at -20°C. Thawed aliquots may be stored at 4°C for up to 4-6 weeks.
4. Medium Supplementation: Add FBS to basal culture medium at a typical final concentration of 5-20%, depending on cell type and experimental requirements. Mix gently by swirling.
5. Storage of Complete Medium: Complete medium supplemented with FBS should be stored at 4°C and used within 2-4 weeks.
Precautions
1. Do not thaw serum at temperatures above 37°C as excessive heat may degrade growth factors and promote precipitate formation.
2. Avoid repeated freeze-thaw cycles; aliquot into single-use volumes after initial thawing.
3. If precipitate forms during storage, centrifuge or filter the serum before use. Precipitate does not indicate product degradation.
4. Heat inactivation may be required for certain cell types or applications but is not necessary for most routine cell culture.
5. For research use only. Not for use in diagnostic or therapeutic procedures. Not for human or animal therapeutic use.
FAQ (Simplified)
Q1: Is heat inactivation necessary?
A1: Heat inactivation is not required for most cell culture applications. It is recommended for specific cell types or assays that are sensitive to complement proteins. Consult published protocols or perform pilot experiments for your specific cell type.
Q2: What is the recommended serum concentration for cell culture?
A2: Typical FBS concentration ranges from 5-20%. Most tumor cell lines grow well in 5-10% FBS, while primary cells may require 10-20%. Optimize for your specific cell type.
Q3: What should I do if precipitate forms in the serum?
A3: Precipitate may form during storage and does not indicate degradation. Centrifuge or filter the serum before use. If turbidity is extensive, contact the supplier.
Q4: How should I store the serum after thawing?
A4: Store thawed serum at 4°C and use within 4-6 weeks. For long-term storage, aliquot into single-use volumes and store at -20°C. Avoid repeated freeze-thaw cycles.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for human or animal therapeutic use.
2. Due to the variable nature of biological research, optimization of serum concentration is recommended for specific cell types and experimental systems.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-1902
Product Name: Premium Fetal Bovine Serum
Size: 500 mL
Suggested Retail Price: CNY ¥3800.00 / USD $380.00 / EUR �456.00 / JPY ¥68400.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
