Polybrene (10 mg/mL), IC-6017

DNA Transfection Enhancer, Sterile Solution

Product Introduction

Polybrene (hexadimethrine bromide) is a cationic polymer widely used as a transfection enhancer to increase the efficiency of retroviral, lentiviral, and plasmid DNA transduction into mammalian cells. Polybrene functions by neutralizing the negative charge repulsion between the cell membrane and viral particles or DNA molecules, facilitating their interaction and subsequent cellular uptake. At a working concentration of 10 mg/mL, this sterile solution is ready-to-use for direct addition to culture medium. This product is supplied as a sterile-filtered solution, ensuring compatibility with sensitive cell culture applications.

Product Features

1. Enhanced Transduction Efficiency: Neutralizes charge repulsion between cells and viral particles or DNA.

2. Ready-to-Use Sterile Solution: Supplied at 10 mg/mL in sterile format for direct use.

3. Broad Application: Compatible with retroviral, lentiviral, and plasmid DNA transfection protocols.

4. Convenient Format: 0.5 mL packaging suitable for routine laboratory use.

Specifications

Size: 0.5 mL

Concentration: 10 mg/mL

Form: Sterile solution

Application: DNA transfection enhancer for retroviral, lentiviral, and plasmid transduction

Storage and Stability

Storage Conditions: Store at -20¡ãC, protected from light. Working aliquots may be stored at 4¡ãC for up to 2 weeks.

Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed. Avoid repeated freeze-thaw cycles.

Protocol (For Reference Only)

Important: The optimal Polybrene concentration should be determined empirically for each cell type, as sensitivity to Polybrene varies between cell lines. Typical working concentrations range from 4-10 µg/mL.

1. Working Solution Preparation: Thaw the Polybrene stock solution at room temperature. Dilute in culture medium to the desired working concentration (typically 4-10 µg/mL).

2. Viral Transduction: Mix viral supernatant with Polybrene to achieve the desired final concentration. Add the mixture to target cells and incubate according to standard transduction protocols.

3. Plasmid Transfection: Add Polybrene to the DNA-transfection reagent mixture at a final concentration of 4-10 µg/mL during cell transfection.

4. Post-Transduction: Replace the Polybrene-containing medium with fresh culture medium after 6-24 hours of incubation, as prolonged exposure may cause cytotoxicity in some cell types.

Precautions

1. Polybrene can be toxic to certain cell lines at high concentrations or with prolonged exposure. Optimize the concentration and incubation time for each cell type.

2. Avoid repeated freeze-thaw cycles; aliquot into single-use volumes upon first thaw.

3. Some cell types (e.g., primary neurons, hematopoietic stem cells) are particularly sensitive to Polybrene. Use lower concentrations or alternative transduction enhancers if cytotoxicity is observed.

4. For research use only. Not for use in diagnostic or therapeutic procedures.

FAQ (Simplified)

Q1: What is the recommended working concentration of Polybrene?

A1: The typical working concentration is 4-10 µg/mL. Optimize for each cell type as sensitivity varies.

Q2: Can Polybrene be toxic to cells?

A2: Yes. Polybrene can be cytotoxic at high concentrations or with prolonged exposure. Replace medium 6-24 hours after transduction.

Q3: How should I store this product?

A3: Store at -20¡ãC. Working aliquots may be stored at 4¡ãC for up to 2 weeks. Avoid repeated freeze-thaw cycles.

Disclaimer

1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.

2. Due to the variable nature of biological research, optimization of working concentration and incubation time is recommended for specific cell types.

3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.

4. Wear appropriate protective clothing and gloves when handling this product.

Ordering Information

Catalog Number: IC-6017

Product Name: Polybrene (10 mg/mL), DNA Transfection Enhancer, Sterile Solution

Size: 0.5 mL

Suggested Retail Price: CNY ¥400.00 / USD $40.00 / EUR €48.00 / JPY ¥7200.00

Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.