DiI-Labeled Low Density Lipoprotein, IC-1373
Product Introduction
This product is low density lipoprotein (LDL) labeled with DiI (1,1''''-dioctadecyl-3,3,3'''',3'''' -tetramethylindocarbocyanine perchlorate), a lipophilic fluorescent carbocyanine dye. LDL is the major cholesterol-carrying lipoprotein in plasma, responsible for transporting cholesterol to peripheral tissues through receptor-mediated endocytosis via the LDL receptor (LDLR). DiI-LDL is widely used as a fluorescent probe for studying LDL receptor-mediated endocytosis, cellular cholesterol uptake, and LDL receptor expression and function. Upon binding to LDL receptors on the cell surface, DiI-LDL is internalized through clathrin-mediated endocytosis and delivered to lysosomes, where the DiI fluorescence can be visualized by fluorescence microscopy or quantified by flow cytometry. DiI-LDL is extensively utilized in atherosclerosis research, lipoprotein metabolism studies, and investigations of receptor-mediated endocytosis pathways. The DiI fluorophore exhibits orange-red fluorescence with excitation/emission maxima of approximately 549/565 nm.
Product Features
1. Fluorescent LDL Probe: DiI labeling enables visualization and quantification of LDL uptake and metabolism.
2. Receptor-Mediated Uptake: Preserves native LDL structure for binding to LDL receptors.
3. Broad Research Utility: Applied in atherosclerosis research, lipoprotein metabolism, endocytosis studies, and LDL receptor functional assays.
4. Compatible with Multiple Platforms: Suitable for fluorescence microscopy, flow cytometry, and cell-based assays.
Specifications
Size: 500 µg
Label: DiI (1,1''''-dioctadecyl-3,3,3'''',3''''-tetramethylindocarbocyanine perchlorate)
Excitation/Emission: ~549 nm / ~565 nm
Source: Human plasma LDL
Storage and Stability
Storage Conditions: Store at 4¡ãC, protected from light. Do not freeze.
Shelf Life: The product is stable for 3 months from the date of manufacture when stored as directed.
Protocol (For Reference Only)
Important: Protect from light. Avoid repeated freeze-thaw cycles. Use aseptic technique for cell culture applications.
1. Working Solution Preparation: Dilute DiI-LDL in serum-free culture medium or PBS to the desired working concentration (typically 5-20 µg/mL).
2. Cell Treatment: Add DiI-LDL working solution to cultured cells. Incubate at 37¡ãC for 2-4 hours for uptake studies. For binding studies, incubate at 4¡ãC for 1-2 hours to prevent internalization.
3. Washing: Remove the DiI-LDL-containing medium. Wash cells 2-3 times with PBS to remove unbound DiI-LDL.
4. Detection: Visualize by fluorescence microscopy using a rhodamine/TRITC filter set, or quantify by flow cytometry in the PE channel.
5. Competition Studies: To confirm LDL receptor specificity, pre-incubate cells with excess unlabeled LDL (e.g., 10-50¡Á excess) before adding DiI-LDL.
Precautions
1. Protect from light during handling and storage.
2. Do not freeze; freezing may cause lipoprotein aggregation and loss of receptor binding activity.
3. Optimize working concentration and incubation time for each cell type.
4. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: How should I store DiI-LDL?
A1: Store at 4¡ãC protected from light. Do not freeze.
Q2: What is the typical working concentration?
A2: Typical working concentrations range from 5-20 µg/mL. Optimize for each cell type.
Q3: How can I confirm LDL receptor-mediated uptake?
A3: Perform competition studies by adding excess unlabeled LDL (10-50¡Á) to block receptor-mediated binding and uptake. Alternatively, use LDL receptor-deficient cells as negative controls.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of staining conditions is recommended for specific cell types.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-1373
Product Name: DiI-Labeled Low Density Lipoprotein
Size: 500 µg
Suggested Retail Price: CNY ¥3680.00 / USD $368.00 / EUR €441.60 / JPY ¥66240.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
