FITC, IC-03308655
Product Introduction
FITC (Fluorescein isothiocyanate) is an amine-reactive fluorescent labeling reagent widely used for conjugating proteins, antibodies, peptides, and other biomolecules. FITC contains an isothiocyanate group that reacts with primary amines (e.g., lysine residues and N-terminal amines) to form stable thiourea linkages. It exhibits bright green fluorescence with excitation and emission maxima at approximately 490 nm and 525 nm, respectively, and is compatible with standard FITC filter sets and 488 nm laser-equipped flow cytometers and fluorescence microscopes. FITC is extensively used in immunofluorescence, flow cytometry, ELISA, protein labeling, and nucleic acid labeling applications.
Product Features
1. Amine-Reactive Labeling: Isothiocyanate group reacts with primary amines to form stable thiourea conjugates.
2. Bright Green Fluorescence: Excitation/emission maxima at ~490/525 nm, compatible with standard FITC channels.
3. Broad Application: Suitable for labeling antibodies, proteins, peptides, and other amine-containing biomolecules.
4. Well-Established Reagent: Widely used in immunofluorescence, flow cytometry, and ELISA.
5. High Purity: Supplied at 98% purity for reproducible labeling.
Specifications
Size: 5 mg / 25 mg / 100 mg
CAS Number: 3326-32-7
Purity: 98%
Molecular Weight: 389.38
Molecular Formula: C21H11NO5S
Excitation/Emission: ~490 nm / ~525 nm
Solubility: DMSO: ¡Ý10 mg/mL; Ethanol: slightly soluble; reacts with water
Storage and Stability
Storage Conditions: Store at 2-8¡ãC, protected from light and moisture, under inert atmosphere.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed.
Protocol (For Reference Only)
Important: FITC is moisture-sensitive and light-sensitive. Prepare solutions freshly before use and protect from light. FITC reacts with water; use anhydrous solvents for stock solutions.
1. Stock Solution Preparation: Dissolve FITC in anhydrous DMSO or DMF to prepare a concentrated stock solution (e.g., 10-50 mM). Prepare fresh before use and protect from light.
2. Protein Labeling: Dilute the protein to be labeled in 0.1 M sodium carbonate/bicarbonate buffer, pH 9.0-9.5, at a concentration of 1-10 mg/mL. Add FITC stock solution at a molar ratio of 5-50:1 (FITC:protein). Incubate at room temperature for 1-2 hours or at 4¡ãC overnight with gentle mixing, protected from light.
3. Purification: Remove unreacted FITC by gel filtration chromatography, dialysis, or ultrafiltration. Determine the degree of labeling by measuring absorbance at 495 nm and 280 nm.
4. Storage of Conjugates: Store purified conjugates at 4¡ãC protected from light in the presence of a preservative for short-term use, or aliquot and store at -20¡ãC for long-term storage. Avoid repeated freeze-thaw cycles.
5. Working Dilution: Determine the optimal working dilution empirically for each application.
Precautions
1. Protect from light and moisture during handling and storage.
2. FITC reacts with water; use anhydrous solvents for stock solutions and prepare fresh before use.
3. Conjugation efficiency is affected by buffer pH, protein concentration, and molar ratio; optimization is recommended.
4. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the reactive group of FITC?
A1: FITC contains an isothiocyanate group that reacts with primary amines on proteins and other biomolecules to form stable thiourea linkages.
Q2: What buffer should I use for protein labeling?
A2: Use 0.1 M sodium carbonate/bicarbonate buffer at pH 9.0-9.5. Avoid buffers containing primary amines such as Tris or glycine.
Q3: How should I store FITC?
A3: Store at 2-8¡ãC protected from light and moisture under inert atmosphere. Prepare stock solutions in anhydrous DMSO or DMF and use freshly.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of labeling conditions is recommended for specific applications.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-03308655
Product Name: FITC
Size: 5 mg / 25 mg / 100 mg
Suggested Retail Price:
5 mg: CNY ¥234.00 / USD $23.40 / EUR €28.08 / JPY ¥4212.00
25 mg: CNY ¥562.00 / USD $56.20 / EUR €67.44 / JPY ¥10116.00
100 mg: CNY ¥675.00 / USD $67.50 / EUR €81.00 / JPY ¥12150.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
