Fmoc-Selenomethionine, IC-1008446
Product Introduction
Fmoc-Selenomethionine is an Fmoc-protected selenomethionine derivative used in solid-phase peptide synthesis (SPPS) for the site-specific incorporation of selenomethionine into peptides and proteins. Selenomethionine is a selenium analog of methionine in which the sulfur atom is replaced by selenium. This substitution enables the use of multi-wavelength anomalous dispersion (MAD) and single-wavelength anomalous dispersion (SAD) techniques in X-ray crystallography for phase determination, as selenium provides strong anomalous scattering signals. Fmoc-Selenomethionine is compatible with standard Fmoc-based SPPS protocols and can be incorporated into peptides for structural biology studies, protein-ligand interaction research, and the development of selenium-containing peptide therapeutics. The Fmoc protecting group is removed under standard piperidine treatment conditions, allowing seamless integration into automated peptide synthesis workflows.
Product Features
1. Fmoc-Protected: Compatible with standard Fmoc-based solid-phase peptide synthesis protocols.
2. Selenium-Containing: Enables MAD/SAD phasing in X-ray crystallography for structural determination.
3. Site-Specific Incorporation: Allows precise placement of selenomethionine residues in synthetic peptides.
4. High Purity: Supplied at 98% purity for reproducible peptide synthesis.
5. Broad Application: Suitable for structural biology, protein engineering, and peptide chemistry research.
Specifications
Size: 1 g / 5 g
CAS Number: 1217852-49-7
Purity: 98%
Molecular Weight: 432.36
Molecular Formula: C20H21NO4Se
Appearance: White to off-white powder
Solubility: DMF: ¡Ý10 mg/mL; DMSO: ¡Ý10 mg/mL; limited solubility in aqueous buffers
Storage and Stability
Storage Conditions: Store at -20¡ãC, protected from light and moisture.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed.
Protocol (For Reference Only)
Important: Protect from light and moisture. Use anhydrous solvents for coupling reactions.
1. Stock Solution Preparation: Dissolve Fmoc-Selenomethionine in anhydrous DMF to prepare a coupling solution at the desired concentration (e.g., 0.3-0.5 M).
2. Solid-Phase Peptide Synthesis: Incorporate Fmoc-Selenomethionine into the growing peptide chain using standard Fmoc SPPS coupling reagents (e.g., HBTU/HOBt, DIC/Oxyma) according to established protocols.
3. Fmoc Deprotection: Remove the Fmoc protecting group using 20% piperidine in DMF according to standard protocols.
4. Cleavage and Purification: Cleave the peptide from the resin and purify by HPLC according to standard procedures. Handle selenium-containing peptides with appropriate precautions.
5. Characterization: Confirm the incorporation of selenomethionine by mass spectrometry and amino acid analysis.
Precautions
1. Protect from light and moisture during storage.
2. Selenium compounds should be handled with appropriate safety precautions; avoid inhalation and skin contact.
3. Use anhydrous solvents for coupling reactions to prevent hydrolysis of activated intermediates.
4. Dispose of selenium-containing waste according to institutional guidelines.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the application of Fmoc-Selenomethionine?
A1: It is used for the site-specific incorporation of selenomethionine into synthetic peptides for X-ray crystallography (MAD/SAD phasing), structural biology, and peptide chemistry research.
Q2: Is Fmoc-Selenomethionine compatible with standard SPPS?
A2: Yes. It is compatible with standard Fmoc-based solid-phase peptide synthesis protocols and can be incorporated using conventional coupling reagents.
Q3: How should I store this product?
A3: Store at -20¡ãC protected from light and moisture.
Q4: What safety precautions should I take?
A4: Handle selenium compounds with appropriate safety precautions, avoiding inhalation and skin contact. Dispose of waste according to institutional guidelines.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of peptide synthesis, optimization of coupling conditions is recommended for specific sequences.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-1008446
Product Name: Fmoc-Selenomethionine
Size: 1 g / 5 g
Suggested Retail Price:
1 g: CNY ¥3500.00 / USD $350.00 / EUR €420.00 / JPY ¥63000.00
5 g: CNY ¥7500.00 / USD $750.00 / EUR €900.00 / JPY ¥135000.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
