HCTU, 330645-87-9,IC-01248577
Product Introduction
HCTU (O-(1H-6-Chlorobenzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate) is a highly efficient uronium-type peptide coupling reagent widely used in solid-phase peptide synthesis (SPPS) and solution-phase amide bond formation. HCTU activates carboxylic acids in the presence of a tertiary base (e.g., DIPEA) to form an active ester intermediate that reacts rapidly with amines to form amide bonds. Compared to HBTU and HATU, HCTU offers faster coupling kinetics and reduced racemization, making it particularly suitable for the synthesis of challenging peptide sequences, including sterically hindered and aggregation-prone peptides. HCTU is compatible with Fmoc-based SPPS strategies and is commonly used in automated peptide synthesizers. Its high reactivity and excellent coupling efficiency make it a preferred coupling reagent for research and production-scale peptide synthesis.
Product Features
1. Highly Efficient Coupling: Faster coupling kinetics and excellent coupling efficiency compared to conventional uronium reagents.
2. Reduced Racemization: Minimizes epimerization during amide bond formation, preserving chiral integrity.
3. Broad Compatibility: Suitable for Fmoc-based SPPS and solution-phase amide synthesis.
4. Excellent Solubility: Freely soluble in DMF, NMP, and DCM for convenient preparation of coupling solutions.
5. High Purity: Supplied at high purity for reproducible peptide synthesis.
Specifications
Size: 500 g / 1 kg
CAS Number: 330645-87-9
Molecular Weight: 413.72
Molecular Formula: C11H15ClF6N5OP
Purity: ¡Ý99%
Appearance: White to off-white powder
Solubility: DMF: ¡Ý100 mg/mL; NMP: ¡Ý100 mg/mL; DCM: ¡Ý50 mg/mL
Storage and Stability
Storage Conditions: Store at 2-8¡ãC under inert atmosphere, protected from light and moisture.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed. For long-term storage, store at -20¡ãC under inert atmosphere.
Protocol (For Reference Only)
Important: Protect from light and moisture. Use anhydrous solvents and inert atmosphere for coupling reactions.
1. Coupling Solution Preparation: Dissolve HCTU in anhydrous DMF or NMP to prepare a coupling solution at the desired concentration (typically 0.3-0.5 M).
2. Solid-Phase Peptide Synthesis: Combine the Fmoc-amino acid, HCTU, and a tertiary base (e.g., DIPEA) in DMF at a molar ratio of 1:1:2 (amino acid:HCTU:base) and add to the resin-bound free amine. Couple according to standard Fmoc SPPS protocols.
3. Deprotection: Remove the Fmoc protecting group using 20% piperidine in DMF before the next coupling cycle.
4. Cleavage and Purification: Cleave the synthesized peptide from the resin and purify by HPLC according to standard procedures.
5. Characterization: Confirm peptide identity and purity by mass spectrometry and HPLC analysis.
Precautions
1. Protect from light and moisture during storage and handling.
2. Use anhydrous solvents and inert atmosphere to prevent hydrolysis of the active ester intermediate.
3. HCTU may cause sensitization by skin contact; wear appropriate protective clothing and gloves.
4. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What are the advantages of HCTU over HBTU?
A1: HCTU offers faster coupling kinetics, improved coupling efficiency, and reduced racemization compared to HBTU, making it particularly suitable for challenging peptide sequences.
Q2: What base is recommended with HCTU?
A2: DIPEA (N,N-diisopropylethylamine) is typically used at 2 equivalents relative to the amino acid to activate the carboxylic acid and neutralize the released acid.
Q3: How should I store HCTU?
A3: Store at 2-8¡ãC under inert atmosphere, protected from light and moisture. For long-term storage, store at -20¡ãC under inert atmosphere.
Q4: Is HCTU compatible with automated peptide synthesizers?
A4: Yes. HCTU is widely used in automated Fmoc-based solid-phase peptide synthesizers and is compatible with standard coupling protocols.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of peptide synthesis, optimization of coupling conditions is recommended for specific sequences.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-01248577
Product Name: HCTU
Size: 500 g / 1 kg
Suggested Retail Price:
500 g: CNY ¥950.00 / USD $95.00 / EUR €114.00 / JPY ¥17100.00
1 kg: CNY ¥1500.00 / USD $150.00 / EUR €180.00 / JPY ¥27000.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
