CellPro™ DNA-Red (UV Nucleic Acid Dye), IC-6009

Product Introduction

CellPro™ DNA-Red is a nucleic acid dye designed for DNA and RNA staining in agarose and polyacrylamide gel electrophoresis. It exhibits spectral properties similar to ethidium bromide (EB), allowing direct substitution in existing protocols without changing filter sets or observation equipment. Standard EB or SYBR filter sets and conventional UV gel transilluminators are compatible, with optimal excitation near 300 nm. The dye is available as a 10,000¡Á concentrated stock solution.

Product Features

1. Low Toxicity: The oil-based, high molecular weight characteristics of CellPro™ DNA-Red prevent it from penetrating cell membranes. Ames test results indicate that its mutagenicity is substantially lower than that of EB.

2. High Sensitivity: Suitable for staining DNA fragments of various sizes, with less interference with nucleic acid migration than SYBR Green I.

3. High Stability: Suitable for agarose gels prepared by microwave or other heating methods. Stable in acidic or alkaline buffers at room temperature and resistant to light.

4. High Signal-to-Noise Ratio: Strong sample fluorescence signal with low background signal.

5. Simple Operation: Like EB, the dye does not degrade during gel casting or electrophoresis. Post-electrophoresis staining requires only 30 minutes with no destaining or washing, and gels can be directly visualized using a UV gel transilluminator.

6. Broad Application: Supports pre-electrophoresis staining (in-gel staining) or post-electrophoresis staining (soak staining). Suitable for agarose or polyacrylamide gel electrophoresis and for staining dsDNA and ssDNA.

7. EB-Compatible Spectral Properties: Standard EB or SYBR filter sets and conventional UV gel transilluminators are compatible, with optimal excitation near 300 nm. However, CellPro™ DNA-Red cannot be fully excited by a 488 nm argon ion laser or visible light of similar wavelength, so imaging systems using such excitation are not recommended. For such instruments, CellPro™ DNA-Green (Cat# IC-6010) is recommended, which has spectral properties similar to SYBR Green I with comparable sensitivity and greater stability.

Specifications

Size: 500 µL

Concentration: 10,000¡Á stock solution

Excitation: Optimal near 300 nm (UV)

Compatible Filters: Standard EB or SYBR filter sets

Application: dsDNA and ssDNA staining in agarose and polyacrylamide gels

Storage and Stability

Storage Conditions: Store at room temperature, protected from light. The 3¡Á working staining solution may be stored at room temperature protected from light until use.

Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed.

Protocol (For Reference Only)

Important: Wear gloves and appropriate protective equipment when handling. Avoid direct exposure to UV light during visualization.

1. In-Gel Staining (Same as EB, Recommended)

1.1 Add CellPro™ DNA-Red nucleic acid dye to the agarose solution during gel preparation (e.g., add 5 µL of CellPro™ DNA-Red 10,000¡Á stock to every 50 mL of agarose solution; scale proportionally).

1.2 Perform electrophoresis according to standard procedures.

Notes:

- This method requires relatively little dye. Approximately 500 µL of dye is sufficient for about 100 gels of 50 mL each.

- Due to the good thermal stability of CellPro™ DNA-Red, it can be added directly to hot agarose solution without waiting for cooling. Shake, vortex, or invert to ensure thorough mixing. Alternatively, add CellPro™ DNA-Red stock to the agarose powder and electrophoresis buffer, then heat in a microwave or by other common methods to prepare the agarose gel. CellPro™ DNA-Red is compatible with all commonly used electrophoresis buffers.

- If bands appear diffuse or separation is unsatisfactory, soak staining is recommended to determine whether the issue is related to the dye. If the problem persists after staining, it is not related to the dye. In that case, try: reducing agarose concentration; using a longer gel; extending gelation time to ensure clear edges; improving loading technique; or selecting soak staining.

- This method is not suitable for pre-cast polyacrylamide gels. For polyacrylamide gels, use soak staining.

2. Soak Staining

2.1 Perform electrophoresis according to standard procedures.

2.2 Dilute CellPro™ DNA-Red 10,000¡Á stock approximately 3,300-fold into 0.1 M NaCl with H₂O to prepare 3¡Á staining solution (e.g., add 15 µL of CellPro™ DNA-Red 10,000¡Á stock and 5 mL of 1 M NaCl to 45 mL of H₂O).

2.3 Carefully place the gel into a suitable container, such as a polypropylene container. Slowly add sufficient 3¡Á staining solution to submerge the gel. Stain at room temperature with agitation for approximately 30 minutes. Optimal staining time varies slightly depending on gel thickness and agarose concentration. For gels containing 3.5-10% acrylamide, staining time typically ranges from 30 minutes to 1 hour, increasing with acrylamide content.

Notes:

- Soak staining requires more dye. A single working staining solution may be reused approximately 3 times.

- 3¡Á CellPro™ DNA-Red staining solution can be prepared in bulk and stored at room temperature protected from light until use.

Special Notes:

- For UV imaging systems, choose CellPro™ DNA-Red. For visible light observation, choose CellPro™ DNA-Green.

- In rare cases, plasmid DNA samples digested with certain enzymes may exhibit smearing and reduced resolution. It is recommended to try both staining methods to determine which is more suitable.

Precautions

1. Although CellPro™ DNA-Red exhibits lower mutagenicity than EB, it should still be handled with appropriate protective equipment and disposed of according to institutional guidelines.

2. Protect from light during storage.

3. Avoid direct exposure to UV light during visualization.

4. For research use only. Not for use in diagnostic or therapeutic procedures.

FAQ (Simplified)

Q1: Can CellPro™ DNA-Red be used as a direct substitute for EB?

A1: Yes. CellPro™ DNA-Red has spectral properties similar to EB, and standard EB filter sets and UV transilluminators can be used without modification.

Q2: Which staining method is recommended?

A2: In-gel staining (same as EB) is recommended for agarose gels due to its simplicity and low dye consumption. Soak staining is recommended for polyacrylamide gels or when in-gel staining produces unsatisfactory results.

Q3: Can the dye be added to hot agarose solution?

A3: Yes. CellPro™ DNA-Red has good thermal stability and can be added directly to hot agarose solution without waiting for cooling.

Q4: Is this dye suitable for imaging systems using a 488 nm laser?

A4: No. CellPro™ DNA-Red cannot be fully excited by a 488 nm argon ion laser or similar visible light. For such systems, CellPro™ DNA-Green (Cat# IC-6010) is recommended.

Disclaimer

1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.

2. Due to the variable nature of biological research, optimization of staining conditions is recommended for specific gel systems and applications.

3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.

4. Wear appropriate protective clothing and gloves when handling this product. Dispose of dye waste according to institutional guidelines.

Ordering Information

Catalog Number: IC-6009

Product Name: CellPro™ DNA-Red (UV Nucleic Acid Dye)

Size: 500 µL

Suggested Retail Price: CNY ¥687.50 / USD $68.75 / EUR €82.50 / JPY ¥12375.00

Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.