2¡Á Fast Taq PCR Master Mix, IC-5209
Product Introduction
2¡Á Fast Taq PCR Master Mix is an optimized 2¡Á concentrated PCR premix formulated with a modified Taq DNA Polymerase that exhibits an amplification speed approximately 4 times faster than conventional Taq DNA Polymerase, reaching up to 15 sec/kb. The formulation is further supplemented with a single-stranded DNA-binding protein, PCR enhancers, and protein stabilizers that work synergistically to improve amplification efficiency and sensitivity. It can amplify fragments up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA. The product is convenient to use: simply add 0.5 reaction volume of 2¡Á Fast Taq PCR Master Mix, primers, and template, and adjust the final volume with ddH₂O. The amplified products carry a single adenine (A) overhang at the 3'''''''' end, allowing direct cloning into T-Vectors. The mix contains a blue electrophoresis tracking dye, enabling direct agarose gel electrophoresis analysis of amplification products without additional loading buffer.
Product Features
1. Fast Amplification: Modified Taq DNA Polymerase enables amplification speeds up to 15 sec/kb, approximately 4 times faster than conventional Taq.
2. Enhanced Efficiency and Sensitivity: Single-stranded DNA-binding protein, PCR enhancers, and protein stabilizers work synergistically to improve amplification performance.
3. Long Fragment Amplification: Capable of amplifying fragments up to 4 kb from complex genomic DNA and up to 5 kb from lambda DNA.
4. Convenient 2¡Á Premix Format: Simply add primers and template, then adjust volume with ddH₂O.
5. Direct T-Vector Cloning: Amplified products carry a 3'''''''' A overhang for direct cloning into T-Vectors.
6. Direct Gel Loading: Contains blue electrophoresis tracking dye for direct agarose gel electrophoresis.
Specifications
Size: 1 mL / 5 mL / 15 mL
Concentration: 2¡Á
Polymerase: Modified Taq DNA Polymerase
Amplification Speed: Up to 15 sec/kb
Maximum Amplicon Size: Up to 4 kb (genomic DNA), up to 5 kb (lambda DNA)
Overhang: 3'''''''' A overhang
Tracking Dye: Blue electrophoresis tracking dye included
Storage and Stability
Shipping Conditions: Low-temperature transport.
Storage Conditions: Store at -20¡ãC.
Shelf Life: More than 2 years from the date of manufacture when stored at -20¡ãC as directed.
Protocol (For Reference Only)
Important: Thaw the Master Mix on ice and mix gently before use. Avoid repeated freeze-thaw cycles.
1. Reaction Setup: Prepare the PCR reaction on ice according to the following typical 50 µL reaction: 25 µL of 2¡Á Fast Taq PCR Master Mix, forward primer (final concentration 0.2-0.5 µM), reverse primer (final concentration 0.2-0.5 µM), template DNA (10-100 ng genomic DNA or 1-10 ng plasmid DNA), and ddH₂O to a final volume of 50 µL.
2. PCR Cycling: Use the following typical cycling conditions: initial denaturation at 94-95¡ãC for 1-3 minutes; 25-35 cycles of denaturation at 94-95¡ãC for 10-15 seconds, annealing at 50-65¡ãC for 15-30 seconds (optimize according to primer Tm), extension at 72¡ãC for 15 seconds per kb; final extension at 72¡ãC for 5 minutes.
3. Analysis: Load the PCR products directly onto an agarose gel for electrophoresis; the blue tracking dye allows direct loading without additional loading buffer.
4. Cloning (Optional): Purify the amplified product and clone directly into a T-Vector according to standard protocols.
Precautions
1. Thaw the Master Mix on ice and mix gently before use. Avoid vortexing.
2. Avoid repeated freeze-thaw cycles; aliquot into single-use volumes if needed.
3. Optimize annealing temperature and cycle number according to primer Tm and template complexity.
4. For GC-rich templates, consider adding additional enhancers or optimizing cycling conditions.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the advantage of this Fast Taq Master Mix?
A1: The modified Taq DNA Polymerase enables amplification speeds up to 15 sec/kb, approximately 4 times faster than conventional Taq, significantly reducing PCR cycling time.
Q2: Can the PCR products be cloned directly?
A2: Yes. The amplified products carry a 3'''''''' A overhang, allowing direct cloning into T-Vectors.
Q3: Can I load the PCR products directly onto a gel?
A3: Yes. The Master Mix contains a blue electrophoresis tracking dye, enabling direct loading without additional loading buffer.
Q4: What is the maximum amplicon size?
A4: Up to 4 kb from complex genomic DNA and up to 5 kb from lambda DNA.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of PCR conditions is recommended for specific templates and primer pairs.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-5209
Product Name: 2¡Á Fast Taq PCR Master Mix
Size: 1 mL / 5 mL / 15 mL
Suggested Retail Price:
1 mL: CNY ¥180.00 / USD $18.00 / EUR €21.60 / JPY ¥3240.00
5 mL: CNY ¥400.00 / USD $40.00 / EUR €48.00 / JPY ¥7200.00
15 mL: CNY ¥980.00 / USD $98.00 / EUR €117.60 / JPY ¥17640.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
