Western Blot Thick Filter Paper, IC-8213
Product Introduction
This product is a thick filter paper specifically designed for Western blot transfer applications. During electrophoretic transfer, filter paper is used to assemble the transfer sandwich, providing uniform pressure and support to ensure close contact between the gel and the membrane, thereby facilitating efficient and uniform protein transfer from the gel to the membrane. This thick filter paper is suitable for both wet (tank) transfer and semi-dry transfer systems, and is compatible with nitrocellulose (NC) and PVDF membranes. The filter paper is manufactured from high-purity cellulose fibers with uniform thickness and consistent absorbency, minimizing interference with transfer efficiency and ensuring reproducible results.
Product Features
1. Uniform Thickness: Consistent thickness ensures uniform pressure and close contact between the gel and membrane during transfer.
2. High Absorbency: High-purity cellulose fibers provide consistent absorbency to facilitate efficient buffer and ion transfer.
3. Low Contamination: Free of impurities that may interfere with protein transfer or downstream detection.
4. Broad Compatibility: Suitable for wet (tank) transfer and semi-dry transfer systems, compatible with NC and PVDF membranes.
5. Convenient Packaging: Available in 100-sheet and 50-sheet formats for different experimental scales.
Specifications
Size: 60 tests per box (120 sheets) / 25 tests per box (50 sheets)
Application: Western blot transfer assembly
Material: High-purity cellulose fiber
Compatible Membranes: Nitrocellulose (NC) and PVDF
Compatible Transfer Systems: Wet (tank) transfer and semi-dry transfer
Storage and Stability
Storage Conditions: Store at room temperature (15-25¡ãC) in a dry environment. Protect from moisture and dust.
Shelf Life: The product is stable for 36 months from the date of manufacture when stored as directed in unopened packaging.
Protocol (For Reference Only)
Important: Handle filter paper with clean forceps to avoid contamination. Ensure complete removal of air bubbles when assembling the transfer sandwich.
1. Preparation: Cut the filter paper to the appropriate size, slightly larger than the gel if desired.
2. Buffer Equilibration: Soak the filter paper in transfer buffer until fully saturated.
3. Transfer Sandwich Assembly: Assemble the transfer sandwich in the following order: sponge or fiber pad, filter paper, gel, membrane, filter paper, sponge or fiber pad. Ensure complete removal of air bubbles between each layer using a roller or glass rod.
4. Electrophoretic Transfer: Perform transfer according to standard protocols for the specific transfer system being used.
5. Post-Transfer: Disassemble the sandwich and proceed with blocking and immunodetection.
Precautions
1. Handle filter paper with clean forceps to avoid contamination.
2. Ensure complete removal of air bubbles when assembling the transfer sandwich to prevent localized transfer failure.
3. Do not reuse filter paper.
4. Store in a dry environment to prevent moisture absorption.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What are the specifications of this filter paper?
A1: This product is available as 50 tests per box (100 sheets) and 25 tests per box (50 sheets).
Q2: What transfer systems is this filter paper compatible with?
A2: It is suitable for both wet (tank) transfer and semi-dry transfer systems.
Q3: Can I reuse the filter paper?
A3: No. Filter paper is designed for single use only.
Q4: How should I store this product?
A4: Store at room temperature in a dry environment, protected from moisture and dust.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
3. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-8213
Product Name: Western Blot Thick Filter Paper
Size: 60 tests per box (120 sheets) / 25 tests per box (50 sheets)
Suggested Retail Price:
60 tests per box (120 sheets): CNY ¥700.00 / USD $70.00 / EUR €84.00 / JPY ¥12600.00
25 tests per box (50 sheets): CNY ¥400.00 / USD $40.00 / EUR €48.00 / JPY ¥7200.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
