Anti-Mouse PD-1 Antibody (RMP1-14), IC-09265163
Product Introduction
Anti-Mouse PD-1 Antibody (RMP1-14) is a monoclonal IgG2a antibody inhibitor against mouse PD-1 that blocks PD-1/PD-L1 signaling. PD-1 (programmed cell death protein 1, CD279) is an inhibitory receptor expressed on activated T cells, B cells, and myeloid cells. Binding of PD-1 to its ligands PD-L1 and PD-L2 delivers inhibitory signals that suppress T cell activation, proliferation, and effector function. Blockade of the PD-1/PD-L1 axis with antibodies such as RMP1-14 restores T cell effector function and enhances anti-tumor immune responses, making it a widely used tool in cancer immunology and immunotherapy research.
Product Features
1. PD-1/PD-L1 Blockade: Blocks PD-1/PD-L1 signaling to restore T cell effector function.
2. Validated Binding Activity: Immobilized Mouse PD-1 Protein (His) at 1 µg/mL binds Anti-Mouse PD-1 Antibody (RMP1-14) with an EC50 of 22.030 ng/mL.
3. In Vivo Anti-Tumor Activity: Enhances the anti-tumor effect of combination therapy in MC38 and B16-hCXCR4 tumor models.
4. Low Endotoxin: <1.0 EU/mg, suitable for in vivo studies.
5. Multiple Applications: Validated for ELISA, flow cytometry, and functional assays.
Specifications
Size: 5 mg / 25 mg
Clone: RMP1-14
Alternative Names: Anti-mouse PD-1 (CD279)
Reactivity: Mouse
Target: PDCD1/PD-1/CD279
Isotype: Mouse IgG2a kappa
Clonality: Monoclonal
Conjugation: Unconjugated
Expression System: CHO
Gene ID: 18566
UniProt ID: Q02242
Molecular Weight: 147.57 kDa (Predicted)
Endotoxin: <1.0 EU/mg
Buffer: Supplied as a sterile solution in a buffered formulation system (e.g., phosphate-, citrate-, or amino acid-based). Please refer to the CoA for lot-specific composition.
Target Activity (IC50): PD-1/PD-L1: 4.05 µg/mL; PD-1/PD-L1: 1.99 µg/mL
Applications: ELISA, FCM, Functional assay
Recommended Isotype Control: Mouse IgG2a kappa, Isotype Control
Storage and Stability
Storage Conditions: Store at low temperature. -20¡ãC for 1 year.
Shipping Conditions: Shipped with blue ice or at ambient temperature.
Shelf Life: The product is stable for 12 months from the date of manufacture when stored at -20¡ãC as directed. Avoid repeated freeze-thaw cycles.
Protocol (For Reference Only)
Important: Centrifuge the vial briefly before opening. Handle under aseptic conditions for in vivo applications.
1. In Vitro Binding Assay: Coat plates with Mouse PD-1 Protein (His) at 1 µg/mL and incubate with serial dilutions of Anti-Mouse PD-1 Antibody. Detect binding using an appropriate secondary antibody. The EC50 is 22.030 ng/mL.
2. Flow Cytometry: Incubate cells expressing mouse PD-1 with the antibody at an optimized concentration, followed by detection with a fluorophore-conjugated secondary antibody.
3. Functional Assay: Use the antibody at an optimized concentration to block PD-1/PD-L1 signaling in cell-based functional assays.
4. In Vivo Studies: In MC38 (1¡Á10⁶) or B16-hCXCR4 (5¡Á10⁵) tumor-bearing mice, administer Anti-Mouse PD-1 Antibody intraperitoneally at 5 mg/kg twice weekly for 2 weeks, alone or in combination with other therapeutic agents. Optimize dosing based on the specific experimental model.
Precautions
1. Store at -20¡ãC for long-term storage. Avoid repeated freeze-thaw cycles.
2. Use appropriate isotype control (Mouse IgG2a kappa) in all experiments.
3. For in vivo studies, maintain aseptic technique and use appropriate vehicle controls.
4. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the mechanism of action of this antibody?
A1: Anti-Mouse PD-1 Antibody (RMP1-14) binds to mouse PD-1 and blocks PD-1/PD-L1 signaling, thereby restoring T cell effector function and enhancing anti-tumor immune responses.
Q2: What is the recommended isotype control?
A2: Mouse IgG2a kappa isotype control is recommended for use as a negative control.
Q3: What applications is this antibody validated for?
A3: This antibody is validated for ELISA, flow cytometry, and functional assays.
Q4: How should I store this antibody?
A4: Store at -20¡ãC for up to 1 year. Avoid repeated freeze-thaw cycles.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of concentrations and dosing is recommended for specific experimental systems.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-09265163
Product Name: Anti-Mouse PD-1 Antibody (RMP1-14)
Size: 5 mg / 25 mg
Suggested Retail Price:
5 mg: CNY ¥3250.00 / USD $325.00 / EUR €390.00 / JPY ¥58500.00
25 mg: CNY ¥12570.00 / USD $1257.00 / EUR €1508.40 / JPY ¥226260.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
