Percoll Cell Separation Solution, IC-1185
Product Introduction
This product is a sterile, colloidal silica-based density gradient medium designed for the isolation and purification of cells, organelles, and viruses. Percoll consists of silica particles (15-30 nm diameter) coated with polyvinylpyrrolidone (PVP), which provides a non-toxic, low-osmolality, and low-viscosity medium suitable for the separation of viable cells and subcellular components.
Percoll is widely used for the isolation of blood cells (lymphocytes, monocytes, granulocytes), subcellular organelles (mitochondria, lysosomes), and viruses. It can be used either as a pre-formed continuous or discontinuous gradient, or mixed with cells to form a self-generating gradient upon centrifugation. The low endotoxin content and ready-to-use sterile formulation make it ideal for sensitive primary cell isolation and subsequent cell culture applications.
Product Features
1. Non-toxic and Biocompatible: PVP coating and low osmolality preserve cell viability and function.
2. Versatile Gradient Formation: Suitable for pre-formed step gradients or self-generating gradients in situ.
3. Low Viscosity: Enables rapid cell separation at low centrifugal forces using short spin times.
4. Easy Removal: The colloidal particles can be easily washed away from the purified cell pellet by simple centrifugation.
5. Sterile and Ready-to-Use: Supplied as a sterile solution to minimize contamination risk.
Specifications
Size: Available in 100 mL and 500 mL
Storage and Stability
Storage Conditions: Store at 4¡ãC to 8¡ãC. Do not freeze as freezing destroys the colloidal structure.
Shelf Life: The product is stable for 60 months (5 years) from the date of manufacture when stored unopened at recommended conditions.
Protocol (For Reference Only)
Important: All steps should be performed under aseptic conditions. Undiluted Percoll should be adjusted to physiological osmolality before use by mixing with 1.5 M NaCl or 10X concentrated medium (9 parts Percoll : 1 part 1.5 M NaCl).
1. Gradient Preparation: Dilute Percoll to the desired density using 0.15 M NaCl or cell culture medium; for continuous gradients, centrifuge at 15,000-30,000 x g for 15-30 minutes.
2. Sample Loading: Carefully layer the cell suspension onto the prepared Percoll gradient.
3. Centrifugation: Centrifuge at 200-800 x g for 15-30 minutes at 4¡ãC using a swing-out rotor without brake.
4. Cell Collection: Harvest the purified cell layer from the appropriate density interface using a sterile pipette or syringe.
5. Washing: Dilute the collected cells with 3-5 volumes of PBS or medium, centrifuge at 300-400 x g for 5-10 minutes, and repeat wash twice to remove residual silica particles.
Precautions
1. Do not freeze. Freezing causes irreversible aggregation of the silica particles.
2. Adjust osmolality before use to prevent cell lysis or shrinkage.
3. Use a swing-out rotor for best band resolution.
4. Turn off the centrifuge brake to avoid disrupting the gradient interface.
5. For research use only. Not for human or animal therapeutic use.
FAQ (Simplified)
Q1: Why did my gradient fail to form?
A1: Check if the Percoll stock was frozen. Freezing destroys gradient-forming properties. Ensure centrifuge brake is off.
Q2: How do I adjust osmolality?
A2: Mix 9 parts of undiluted Percoll with 1 part of sterile 1.5 M NaCl or 10X concentrated buffer/media.
Q3: What is the density range achievable?
A3: Undiluted Percoll has a density of approximately 1.130 g/mL. It can be diluted to create gradients from 1.0 to 1.130 g/mL.
Q4: Can Percoll be sterilized?
A4: The product is supplied sterile. Do not autoclave as it will degrade the colloidal structure. Filter sterilization is not recommended as it may clog membranes.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of conditions using a small-scale pilot experiment is strongly recommended before processing valuable samples.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-1185
Product Name: Percoll Cell Separation Solution
Size: 100 mL / 500 mL
Price:
100 mL: CNY ¥1950.00 / USD $195.50 / EUR €234.60 / JPY ¥35190.00
500 mL: CNY ¥8500.00 / USD $850.00 / EUR €1020.00 / JPY ¥153000.00
2023 Version
