Poly-L-Lysine Solution (10X), IC-2121
Product Introduction
This product is a 10X concentrated sterile-filtered solution of Poly-L-Lysine (PLL), a synthetic positively charged homopolymer of the amino acid L-lysine. Poly-L-Lysine is widely used as a coating reagent to enhance cell adhesion and attachment to tissue culture plasticware, glass coverslips, and microfluidic devices. The polycationic nature of PLL facilitates electrostatic interaction with the negatively charged surface of cell membranes and culture substrates, forming a stable monolayer that promotes cell spreading, attachment, and differentiation. This 10X concentrated formulation allows convenient dilution to 1X working solution with sterile water or buffer, providing a cost-effective and versatile solution for routine cell culture applications. It is suitable for coating a broad range of surfaces including tissue culture plates, chamber slides, coverslips, and microelectrode arrays, and is compatible with many adherent cell types including primary neurons, glial cells, fibroblasts, epithelial cells, and various transformed cell lines.
Product Features
1. Enhanced Cell Adhesion: Provides a uniform positively charged surface that promotes rapid and stable attachment of adherent cells, particularly beneficial for primary cells and neurons.
2. 10X Concentrated Formulation: Allows cost-effective storage and convenient dilution to 1X working concentration as needed for specific experimental requirements.
3. Sterile-Filtered and Ready-to-Use: Supplied as a sterile-filtered solution requiring only dilution before application to culture surfaces.
4. Broad Substrate Compatibility: Suitable for coating tissue culture polystyrene, glass coverslips, microfluidic channels, and biochip surfaces.
5. Versatile Application: Effective for culturing primary neurons, astrocytes, fibroblasts, endothelial cells, and many transformed cell lines that exhibit poor adhesion to untreated surfaces.
Specifications
Size: 10 mL (1 bottle)
Formulation: 10X Concentrate (0.1% w/v in sterile deionized water)
Molecular Weight: 70,000-150,000 Da (typical range)
Storage and Stability
Storage Conditions: Store at 4¡ãC. Protect from light. Do not freeze.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed. After opening, use within 3 months when handled aseptically.
Protocol (For Reference Only)
Important: Use aseptic technique when handling the sterile solution. Prepare 1X working solution freshly for optimal coating performance.
1. Preparation of 1X Working Solution: Dilute the 10X Poly-L-Lysine Solution 1:10 with sterile tissue culture grade water or sterile PBS to obtain a 0.01% (w/v) 1X working solution. Mix gently by swirling or inversion.
2. Surface Coating of Culture Vessels: Add sufficient 1X working solution to completely cover the culture surface (typically 0.5 mL per well of a 24-well plate, 1-2 mL per well of a 6-well plate, or 3-5 mL per T25 flask). Incubate at room temperature for 1 hour or at 37¡ãC for 30 minutes.
3. Aspiration and Washing: Aspirate the Poly-L-Lysine solution completely and rinse the coated surface 2-3 times with sterile tissue culture grade water or PBS to remove excess unbound PLL.
4. Drying: Allow the coated surface to air dry completely in a sterile biosafety cabinet with the lid slightly ajar for 1-2 hours. For urgent use, drying can be accelerated by brief exposure under UV light in the biosafety cabinet.
5. Cell Seeding: Once dry, seed cells onto the coated surface in appropriate culture medium and incubate under standard culture conditions. Coated plates may be used immediately or stored at 4¡ãC for up to one week wrapped in sterile packaging.
Precautions
1. Do not freeze the solution as freezing may cause polymer aggregation and loss of coating efficiency.
2. Aspirate the coating solution completely and wash thoroughly to remove excess unbound PLL, as residual free PLL in the culture medium may be cytotoxic to certain cell types.
3. The optimal coating concentration and incubation time may vary for different cell types; optimization is recommended for specific experimental systems.
4. For highly sensitive primary neuronal cultures, consider using freshly coated surfaces and avoid prolonged storage of coated plates.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the difference between Poly-L-Lysine and Poly-D-Lysine?
A1: Poly-L-Lysine is a naturally occurring isomer that can be degraded by cellular proteases, whereas Poly-D-Lysine is resistant to enzymatic degradation and may provide longer-term coating stability. Poly-L-Lysine is generally preferred for short-term culture applications where biocompatibility and mild coating are desired.
Q2: How long can I store coated plates before use?
A2: Coated and dried plates may be stored at 4¡ãC wrapped in sterile packaging for up to one week. For optimal performance, especially with sensitive primary cultures, freshly coated surfaces are recommended.
Q3: Can I use this coating solution for suspension cell types?
A3: Poly-L-Lysine is designed to promote adhesion of adherent cell types. It is not typically required for suspension cells, although it can be used to immobilize suspension cells onto coverslips for imaging applications.
Q4: What should I do if cells still detach despite Poly-L-Lysine coating?
A4: Increase the coating concentration (e.g., try 0.05% final), extend the incubation time to overnight at 4¡ãC, or consider using alternative coating reagents such as poly-D-lysine, collagen, fibronectin, or laminin depending on the specific cell type requirements.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of coating conditions is recommended for specific cell types and experimental applications.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-2121
Product Name: Poly-L-Lysine Solution (10X)
Size: 10 mL
Price: CNY ¥600.00 / USD $60.00 / EUR €72.00 / JPY ¥10800.00
2023 Version
