Recombinant Human IL-23 Protein, CYT-Q9NPF7
Product Introduction
Interleukin-23 (IL-23) is a heterodimeric cytokine composed of the p40 subunit of IL-12 that is disulfide-linked with a p19 subunit unique to IL-23. IL-23 interacts with IL-12Rβ1 and a β2-like receptor subunit with STAT4 binding domain, IL-23R. IL-23 is secreted by activated dendritic cells and is involved in the inflammation process and associated with autoimmune diseases. IL-23 induces proliferation of memory T cells, induces moderate levels of IFN-γ, and is produced by naive and memory T cells. IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance, and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines. IL-23 interacts with the receptors IL-12Rβ1 and IL-23R to activate the Jak-Stat signaling cascade. IL-23 has been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic research.
Product Features
1. Authentic Heterodimeric Structure: Consists of two subunits, p19 (170 amino acids) and p40 (306 amino acids), with native disulfide linkage.
2. High Purity: ≥95% by SDS-PAGE gel and HPLC analyses.
3. Biologically Active: Determined by its ability to stimulate the proliferation of HEK-Blue™-IL-23 reporter cells.
4. Insect Cell Expression: Produced in insect cells for proper post-translational processing.
5. Low Endotoxin: <1 EU/µg.
Specifications
Size: Available in 2 µg and 10 µg
Species: Human
Expression System: Insect cells
Molecular Weight: 53.5 kDa (heterodimer)
Amino Acid Sequence:
p19 subunit: RAVPGGSSPA WTQCQQLSQK LCTLAWSAHP LVGHMDLREE GDEETTNDVP HIQCGDGCDP QGLRDNSQFC LQRIHQGLIF YEKLLGSDIF TGEPSLLPDS PVGQLHASLL GLSQLLQPEG HHWETQQIPS LSPSQPWQRL LLRFKILRSL QAFVAVAARV FAHGAATLSP
p40 subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
Purity: ≥95% by SDS-PAGE and HPLC
Biological Activity: Determined by its ability to stimulate the proliferation of HEK-Blue™-IL-23 reporter cells
Endotoxin: <1 EU/µg
Form: Lyophilized
Conjugate: Unconjugated
Purification: Purified
Preservative: No preservative
Storage and Stability
Storage Conditions: Store at -20°C.
Shipping Conditions: Ambient temperature. Upon receipt, store immediately at the recommended temperature.
Protocol (For Reference Only)
Important: This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis.
1. Reconstitution: Reconstitute as recommended in the lot-specific Certificate of Analysis. Briefly centrifuge the vial prior to opening to bring the contents to the bottom.
2. Aliquoting: Apportion stock solutions into working aliquots and store at ≤ -20°C. Avoid repeated freeze-thaw cycles.
3. Activity Assay: The biological activity is determined by the ability to stimulate proliferation of HEK-Blue™-IL-23 reporter cells.
4. Working Concentration: Optimal working concentrations should be determined empirically for each cell type and experimental system.
Precautions
1. Store at -20°C upon receipt.
2. Refer to the lot-specific Certificate of Analysis for detailed reconstitution and storage instructions.
3. Avoid repeated freeze-thaw cycles.
4. IL-23 is a potent immunomodulatory cytokine; handle with appropriate safety precautions.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the structure of IL-23?
A1: IL-23 is a 53.5 kDa heterodimeric protein consisting of two subunits: p19 (170 amino acids) and p40 (306 amino acids), disulfide-linked to form the biologically active cytokine.
Q2: How does IL-23 function in immune responses?
A2: IL-23 plays a central role in the induction, expansion, maintenance, and downstream effector functions of Th17 cells, which upregulate neutrophil chemokines and various pro-inflammatory cytokines.
Q3: What is the difference between IL-23 and IL-12?
A3: IL-23 and IL-12 share the p40 subunit, but IL-23 contains a unique p19 subunit rather than the p35 subunit found in IL-12. Both interact with IL-12Rβ1, but IL-23 signals through IL-23R rather than IL-12Rβ2.
Q4: How should I store this product?
A4: Store at -20°C. Refer to the lot-specific Certificate of Analysis for detailed storage and handling instructions.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of working concentrations is recommended for specific cell types and experimental systems.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: CYT-Q9NPF7
Product Name: Recombinant Human IL-23 Protein
Size: 2 µg / 10 µg
Suggested Retail Price:
2 µg: CNY ¥1500.00 / USD $150.00 / EUR �180.00 / JPY ¥27000.00
10 µg: CNY ¥3900.00 / USD $390.00 / EUR �468.00 / JPY ¥70200.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
