TBS Buffer (Powder), IC-9002
Product Introduction
This product is a TBS (Tris-Buffered Saline) buffer in powder form, designed for convenient preparation of Tris-buffered saline solution for use in Western blot, ELISA, immunohistochemistry (IHC), and other immunodetection techniques. TBS buffer provides a stable pH environment and physiological ionic strength, making it suitable as a wash buffer, antibody dilution buffer base, and blocking buffer base. The powder format offers advantages in storage, shipping, and shelf life compared to liquid buffers, and eliminates the need for weighing individual buffer components. Each pouch is pre-measured to prepare 1 L of 1¡Á TBS working solution by simple dissolution in distilled water.
Product Features
1. Convenient Powder Format: Pre-measured pouches eliminate weighing and reduce preparation time.
2. Ready-to-Dissolve: Simply dissolve one pouch in 1 L of distilled water to prepare 1¡Á TBS working solution.
3. Stable pH and Ionic Strength: Provides consistent buffering capacity for immunodetection applications.
4. Long Shelf Life: Powder format ensures extended stability compared to liquid buffers.
5. Versatile Application: Suitable as wash buffer, antibody dilution base, and blocking buffer base for Western blot, ELISA, and IHC.
Specifications
Size: 1 L per pouch / 10 ¡Á 1 L per pack
Form: Powder
1¡Á Working Solution: Tris-buffered saline, pH 7.4-7.6 (after dissolution)
Application: Western blot, ELISA, IHC, and other immunodetection techniques
Storage and Stability
Storage Conditions: Store at room temperature (15-25¡ãC) in a dry environment. Keep pouches sealed until use.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed in unopened packaging.
Protocol (For Reference Only)
Important: Use distilled or deionized water for dissolution. Ensure complete dissolution before use.
1. Working Solution Preparation: Open one pouch and empty the contents into a clean container. Add approximately 800 mL of distilled water and stir until completely dissolved. Adjust the final volume to 1 L with distilled water. Mix thoroughly.
2. pH Verification (Optional): Verify the pH of the 1¡Á TBS working solution is between 7.4 and 7.6. Adjust with HCl or NaOH if necessary.
3. Storage of Working Solution: Store the 1¡Á TBS working solution at room temperature or 4¡ãC and use within 1-2 weeks. Discard if turbidity or contamination is observed.
4. Application: Use as a wash buffer for Western blot, ELISA, or IHC, or as a base for preparing antibody dilution buffer or blocking buffer.
Precautions
1. Keep pouches sealed until use to prevent moisture absorption.
2. Use distilled or deionized water for dissolution.
3. Store the prepared 1¡Á TBS working solution at room temperature or 4¡ãC and use within 1-2 weeks.
4. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: How do I prepare 1¡Á TBS from the powder?
A1: Dissolve one pouch in approximately 800 mL of distilled water, then adjust the final volume to 1 L. Mix thoroughly.
Q2: Can I add Tween 20 to prepare TBST?
A2: Yes. Add Tween 20 to a final concentration of 0.05-0.1% (v/v) to prepare TBST for Western blot washing.
Q3: How should I store the powder?
A3: Store at room temperature in a dry environment. Keep pouches sealed until use.
Q4: How long can I store the prepared working solution?
A4: Store the 1¡Á TBS working solution at room temperature or 4¡ãC and use within 1-2 weeks.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
3. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-9002
Product Name: TBS Buffer (Powder)
Size: 1 L per pouch / 10 ¡Á 1 L per pack
Suggested Retail Price:
1 L per pouch: CNY ¥20.00 / USD $2.00 / EUR €2.40 / JPY ¥360.00
10 ¡Á 1 L per pack: CNY ¥200.00 / USD $20.00 / EUR €24.00 / JPY ¥3600.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
