Nuclease-Free Water (Non-DEPC Treated), IC-5311

Product Introduction

This product is high-purity nuclease-free water that has not been treated with DEPC (diethyl pyrocarbonate). It is specifically designed for molecular biology applications where the absence of RNases and DNases is critical for preserving nucleic acid integrity. The water is purified through deionization, distillation, and ultrafiltration to remove nucleases, nucleic acids, and other contaminants, and is certified free of detectable RNase, DNase, and protease activity. Unlike DEPC-treated water, this product does not contain residual DEPC or its degradation byproducts, making it suitable for applications where DEPC may interfere with sensitive enzymatic reactions or downstream analyses. It is suitable for RNA and DNA applications including reverse transcription, PCR, qPCR, in vitro transcription, nucleic acid dilution and storage, and enzymatic reactions.

Product Features

1. Nuclease-Free Certified: Tested negative for RNase, DNase, and protease activity.

2. Non-DEPC Treated: Contains no residual DEPC or degradation byproducts, suitable for DEPC-sensitive applications.

3. High Purity: Processed through deionization, distillation, and ultrafiltration to remove nucleic acids and contaminants.

4. Ready-to-Use: Supplied sterile and ready for immediate use without additional treatment.

5. Versatile Application: Suitable for reverse transcription, PCR, qPCR, in vitro transcription, nucleic acid dilution and storage, and enzymatic reactions.

Specifications

Size: 100 mL / 500 mL

Quality Control: Tested for absence of RNase, DNase, and protease activity

Treatment: Non-DEPC treated

Sterility: Sterile

Storage and Stability

Storage Conditions: Store at room temperature (15-25¡ãC). Use aseptic technique when handling to prevent nuclease contamination.

Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed in unopened packaging. After opening, use within 6 months when handled aseptically.

Protocol (For Reference Only)

Important: Use aseptic technique and wear gloves when handling to prevent nuclease contamination from skin and laboratory surfaces. Aliquot into smaller volumes for frequent use to minimize contamination risk.

1. RNA Reconstitution and Dilution: Use Nuclease-Free Water to reconstitute RNA pellets following precipitation or column purification, and to dilute RNA samples to desired concentrations for downstream applications.

2. cDNA Synthesis: Use Nuclease-Free Water as the diluent for preparing RNA templates, primer mixes, and master mixes in reverse transcription reactions.

3. PCR and qPCR Setup: Use Nuclease-Free Water as the aqueous component for preparing PCR and qPCR reaction master mixes, adjusting reaction volumes, and diluting primers and probes.

4. In Vitro Transcription: Use Nuclease-Free Water for preparing transcription reaction mixes, diluting RNA polymerases, and resuspending in vitro transcribed RNA products.

5. Enzymatic Reaction Preparation: Use Nuclease-Free Water as the universal diluent for nuclease-sensitive enzymatic reactions including restriction digests, ligations, and nucleic acid labeling procedures.

Precautions

1. Use only certified nuclease-free pipette tips and tubes when handling this product; standard laboratory plasticware may introduce RNase contamination.

2. Aliquot the required volume into a separate sterile tube rather than directly pipetting from the stock bottle to minimize contamination risk.

3. Wear clean gloves and change them frequently when working with RNA samples and this product.

4. This product is non-DEPC treated; for applications requiring DEPC-treated water, use a DEPC-treated product instead.

5. For research use only. Not for use in diagnostic or therapeutic procedures.

FAQ (Simplified)

Q1: What is the difference between DEPC-treated and non-DEPC treated nuclease-free water?

A1: DEPC-treated water uses diethyl pyrocarbonate to inactivate RNases, followed by autoclaving to remove residual DEPC. This product is not DEPC-treated and contains no residual DEPC or degradation byproducts, making it suitable for applications where DEPC may interfere with sensitive enzymatic reactions.

Q2: Is this product suitable for RNA work?

A2: Yes. This product is certified nuclease-free and suitable for RNA applications including reverse transcription, in vitro transcription, and RNA storage.

Q3: How should I store and handle this product to maintain nuclease-free status?

A3: Store at room temperature and handle using aseptic technique. Always use certified nuclease-free pipette tips and tubes. Aliquot working volumes into separate sterile tubes to avoid repeated pipetting from the stock bottle.

Q4: Can I use this product for cell culture?

A4: No. This product is intended for molecular biology applications only and is not a tissue culture grade reagent. For cell culture applications, use a dedicated tissue culture grade water.

Disclaimer

1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.

2. Due to the variable nature of biological research, maintenance of nuclease-free technique is the responsibility of the user.

3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.

4. Wear appropriate protective clothing and gloves when handling this product.

Ordering Information

Catalog Number: IC-5311

Product Name: Nuclease-Free Water (Non-DEPC Treated)

Size: 100 mL / 500 mL

Suggested Retail Price:

100 mL: CNY ¥220.00 / USD $22.00 / EUR €26.40 / JPY ¥3960.00

500 mL: CNY ¥650.00 / USD $65.00 / EUR €78.00 / JPY ¥11700.00

Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.